Inside the NZ RNA Platform: Adrienne explores RNA production
23 Video Views·Sep 12, 2026
Adrienne explored the NZ RNA Platform and learned about the RNA production process, spanning sequence design, synthesis, and purification to quality control. The tour provided her with a clear understanding of modern RNA technology, rigorous standards, and potential applications in medicine and research.
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Video Summary
AI GeneratedThis video demonstrates two critical stages of RNA processing: the final washing of an RNA pellet using a centrifuge to remove ethanol and the subsequent purification of synthesized RNA using an FPLC machine to eliminate contaminants.
So this is, we're on to the second wash step of the RNA pellet.
So what's in there is 70% ethanol, so we have to remove that from the tube.
It spins at a really high speed.
So what it does is it removes all the liquid from the side of the walls of the tube and the cap
and it brings it all down to the bottom.
And it concentrates any pellets we have or any flecks of RNA in there.
Would you like some?
No, so if it doesn't really pellet properly, you just have to keep spinning it.
Alright, Addy, here we have the FPLC machine.
So here we're trying to purify our RNA after we've synthesized it over there.
And the way we do it is we run it over these columns which bind the RNA
and then subsequently we elute them from the columns and they're all purified,
get rid of all the contaminants.
And so I'm just going to set it up here.
So here we have a tube containing the RNA.
And then we load it into this machine here.
We just put the line in there.
Yes, so this is already in that salt buffer that makes sure that it's binding.
And then I just need to set up the method here.
Load that in, make sure that the A1 is in that corner.
And then close that again.
And then it should be ready to go.
So you should always check that the tube is all the way at the bottom,
else it's going to suck it up until it hits the air
and then that air is going to stop it from loading more.
And then it will switch to these bottles,
which are then going to wash the column.
And then once the column is washed enough,
then it goes into that buffer that elutes, drags the RNA off.
All right, thank you.