Some analytical techniques ||11th Chemistry || Chapter 3 Part-5 adsorption Partition Chromatography

Because you get marks for writing How to write and how your notes were made I am writing down all that data for you. Columns are used for the zoning phase And what do you do to Mikhser? Separate them And you must do it very well What needs to be done here? Just add it. I want to watch the entire video cut-to-cut. With full focus Hello, kids! How are you all? So kids, in your life They were taught like children, this entire chapter was purely theoretical. And it depends on the practicals. So what am I doing here? I am teaching you the practical aspects first. After that, cut-to-cut, one by one, points will come to you How are you writing your exam? Because if you're writing, or even if you're just watching the whole video with full attention, and if you're new to this channel, then definitely subscribe to this channel and watch its previous parts, and then come back to this part, why? Because the kids, I've made chemistry here very easy, or rather, as clear as butter, so let's go, kids, I'll quickly tell you about this Part Number Five. So here, we'll read a few things. Paper chromatography and both paper chromatography are absolutely fixed in your exam, so from this, the questions If the child had been fixed in your exam, your attention should have been entirely on me. So let's get started with this concept and see what's in it. So, this is our Part No. 5, Basic Analytical Technique, and Chapter No. 3, and the first concept—here comes the child. Look, in chromatography, we have already learned a lot of things. If I tell you the concept, then using chromatography, we can separate two or more mixtures. I mean, suppose you've mixed two mixtures together, or mixed two liquids together, or mixed two mixtures together. So how will the address be determined? Chromatography is a technique whereby we can do what with substances? If we can separate, then today we will read the part on that, which is the first topic: our adsorption chromatography. So first, let's look at this. You must have heard, children, about absorption—have you heard about it? Tell me. absorption means that if I take a beaker and pour water into it, and let's assume here that I have You poured in cotton, right? What did you pour in? Cotton—what will cotton do to this water? Look here. It will go inside Cotton if this molecule goes inside Cotton. So, when we say absorption, now you know what absorption is. Lia Kotin Lia, and you've hidden her inside the water, whose inside? What will Tom do in the bar? Will he go inside Cutan's? What will Roo do? It will absorb all those water molecules within itself, so that is called What is this Vod? We have to read this, so let's see what Atjopshn is. That's right, isn't it? If we don't realize it, just look, let's assume Just accept this in the case. Let's assume this is one piece of cotton. And these are water molecules. What are these molecules of water? If it stays on the surface of this, it cannot get inside. It cannot go inside and remains only on its surface. So that is called word adsorption. What do we call this? Adsorption I mean, you understand one thing very clearly, and that one thing makes everything else clear. if water molecules go inside someone One cannot say that. But if cotton's How do cotton molecules hold water inside themselves? So that is.arange as word adsorption But if the same water molecules belong to a child Now we will see, we have already studied adsorption. But what is adsorption chromatography? Today we will see that. Let's clear up a few things first: adsorption Is chromatography easy? And how many types are there in it? The first topic, meaning the first point, is that it is based on differential adsorption. There is a differential adsorption based on which this has been developed, okay, this Points are not valuable to us; it's just important for you to know the things. Different solutes are adsorbed to varying extents on the stationary phase. I already told you a long time ago about the stationary phase; if you mean the previous one. In the video, what is the stationary phase? When you take the filter paper, that's correct. The filter paper you place inside the mobile face or rather the chromatography paper, also known as Whatman filter paper, is ours is the stationary phase, or if I wanted to use another word, then you When you load the mixer, is that your stationary phase? What exactly is the stationary phase? It depends on the stationary phase as to where your solute will be. It will adsorb, how much will it adsorb, or will it adsorb at all, okay, hey kids You know, look, there was some stationery phase in this series that we used to use. Inside chromatography They spot somewhere here. So, how good is the spot's capability now? Then it will stop. What will happen to this torn, torn, torn, torn? The upper side will move. If I keep any liquid here So it strikes the spot. What will he do with him? Because he's doing up-down why Possibly one who is a mikhshar Your stationery is being excessively used. It's getting too sticky. It might not be the second one that's stuck. He no longer has the ability to form a bond with the stationary phase. We'll take a good look at it once again, and in the next video, we'll find out. She went by, and it's evident even in a video nearby, and far away, it's not clearly audible, but from the gaze, we will understand. Your body also undergoes this universal chromatographic process, so what is the tide? Our tide is also the tide. The chromatograph's head becomes a stationary face, about which a coating is applied. On this tractional face, we are here for the tractional face And what do you do with the mixture, Shalo, let's see how Shalo sees it. You may see a burette here, but we will call it a column. Now, there's no kid above this column, man. Just stay here a bit, okay? Give me a little time, please. If I don't explain, how will you understand? So I'm making just one request of you. that you're constantly glued to your mobile phone and watch my videos so much, I'm currently sharing this concept with you Any three components can be mixed in here, that's fine, if you can't see it I'll fix this and make it even better. Wait here for a minute, I'll get the beaker. What should I do with this, since my ABC mixer is over this silica water, what should I do? I'll insert it, meaning I'll put it inside, okay, and I'll place it very nicely within it. What needs to be done is to take cover. You'll dodge this, kid. So you have this abc is here, this is for you right here It will accumulate. Because down below is your silly jail. Your silly's jail is here. Now, after A-B-C solvent arrived here, what has happened? What happened? It's been collected. You need to use the mobile face, not the facial expressions, for the face. I've had too much of that yummy love stuff. What is your mobile phase? What is your mobile phase? He will come here today. Will he come or won't he come? Now listen carefully, child Listen carefully to what I'm saying, all of you Listen carefully: this is silica gel. Your gel is also in the silica gel. And the formation is of the slurry type. So this slow-moving mobile phase here A B C The solvent of whom you Separate it The mixture that you have, which you need to separate Three mixtures mixed among themselves They are all gathered together in one. What will happen after some time? So when the baby starts coming down When the baby starts coming down So, in this C too It could be possible. Together with Silica District It's going very well with the attachment. Malo Mikshar's molecules are And this is yours Silicon Valley's molecules Whoever establishes good bonding will be able to go down quickly, won't they? You won't be able to get away if you keep repeatedly pressing the solve button. Because the title will remain tied with him. It will take him a little time to go down there. But there might be some such mixer in it. Who haven't formed such a good bond with him If you're setting it up, then whenever you run the solvent So, what will that mobile face do? It could be a mobile face related to the one with letter B. And those with C, if together with this silica gel And the bonding isn't forming properly. So, what will the C guy do about the mobile face? He'll come down very fast, right? So, as this will come down very fast Okay? He will come down with it below. And place a beaker below. And in the same beaker First, take this out. Later, after some time, even more You have to keep reapplying mobile face bar. Then this will come into the second beaker. mixed Assume A has arrived in the first one. So in this In the first one, C will come. C, come down quickly, you're doing well. What will happen to you with the C's? Shalvand will also come today. So you time it for him Solvents can evaporate. You can collect the solute. You can collect the B-type ones. Take it into the second beaker. Also transfer the one from A to the other beaker. AC Apico, this is for you column chromatography Inside adjustment chromatography First, the tapik thaa column chromatography, this will give you Then it will be very clearly understood. I called the children, but I was supposed to call you. I'll tell you the concept, I'll show you cut to cutลาด I'll write even the point here, so let's go Let's start writing our notes, everyone. First point Looking at the point the mixture along with the mobile phase slowly moving down the column As soon as you input the mobile phase, the mixture your abc which mixture in which solid Do it slowly, slowly downward. With mobile face And let's look at the next point We are the ones who are quick and nimble. salute gets adsorbed on the stationary face if the guy with the malo is very Even the one who eats it, no one knows who it is, yet it sticks to everyone. The one that is strongly adsorbed will remain on top. The weekly ads will drop sharply immediately. Three, the ones you took, are correct. And this is now known as Austria. The one who is not getting properly attached. He will go to the desert. The firecracker will explode downward. Alright, and we'll elevate it later. We will take out the letters one by one, separately. What is the next point? In separate beakers Taking components A, B, C, I'm on my way I'm coming, Mikashar. And take it out, evaporate it, and separate it, I mean. Alright, let's go Everyone has been taken away. What do they all keep saying? Column chromatography is not understood by everyone Come here, you big one. Now, chromatic aberration is the second flaw of chromatic optics, the first being spherical aberration. Yahra Your entire attention should be on me, not on Bilalpul. This is my beaker. And in the beaker is your mobile phone. Now you will surely understand the mobile face recognition system. Right on What do you have here, this Mobile Phase Mobile phase is used To run any mixture So in Thin Layer Chromatography We will use a plate. Will the child use one? We will use the plate, OK By doing so, one plate remains with us. Now above this plate The child gets coated on top of this plate. What is on top of this plate? It's a minute coating. I'll take a good brush and coat it with this. Okay? Let's go. A minute, this is completely wrong. In this, you have been quoting it Grandma, what can I do from this jail or Alimunya jail? We apply the coating, so its glass, Now you understand the things, think about it and go. Now, all that remains is to do this. It works for our endies just as it does for Hazelult. The base is dolomite; it is a siliceous country. Coating, now in our memory Working on the cement What will we do, absolutely resolving vidic yadi Random t In this beaker, we have two types of mixtures present. A and B Alright They remain dissolved in an appropriate solvent. What do you need to do with this now? Need to take a capillary sample and from the capillary Draw capillary from the pot And what needs to be done with the capillary? This mischievous one must be removed. And what to hit above 2 cm? And you have to put it into this mobile phase But you'll have to keep these things in mind, kid that when you will put the stationary phase inside this mobile phase So, this dot inside the mobile phase does not go deep, it remains on the surface, the dot that is mixed It will all go into that one, okay? Clear now. Now listen carefully, in a little while If he creates another good bond, the bobail phase will go up very quickly with it. Okay, clear, everyone understands. So that is called what? The child thin layer chromatography, what do they call it? Teach me. Thirley chromatography Everyone understood. What is thin-layer chromatography? Okay, Hua's here We are advancing step by step, point by point. Let's write down the points and see. Because we also need to thoroughly understand the points To understand if we haven't grasped the point So, will the data be cleared or not? You have performed thin-layer chromatography Silica District Vaudho will now be good, so he'll take care of the pot and stay. You will receive a mobile press kit for the UP Baran Baram disease awareness campaign. Now what are you going to do, look? The flip keeps me on the mobile phase, stay attached, I will remain on top of the phase. Don't keep dipping them in the same place; everything else will follow along in that same spot. That spot will be wiped out by a chemical that will go right into it. The mobile phase rises, carrying the components of the mixture along with it. Now the mobile phase run will begin, and the mixture present will start moving along with it. They move upward to different distances depending on the degree of adsorption and thus become separated. Now, observe how that move will be made — it will be made precisely because of how much it is being adsorbed with the stationary phase. So, if the interaction with the mobile phase is weak, the compound will move more, right? That's the point here, and the next point is... If the components are colored, they appear as separate spots on the plates. If they remain colored, you will see distinct spots. But if the components are not colored, yet possess the property of fluorescent material—understood? They won't do anything at first, but once they receive light, they'll absorb it and then glow. Right? If such a phenomenon occurs. If the colorless component is forming a spot that we cannot see, we can visualize it under a UV chamber or using iodine vapors, right? Because iodine vapors adhere to the components and the spot appears. Look, the spot has arrived, but you can't see it. So, go to the iudin chamber, meaning, take a baker, place the iudin inside it, and then, as they say in the stationary face, take that spot which you can't see and bring it along somehow. It'll be over, man, my Mikhser has gone all the way here, he's gone cold. After that, Damiano, Acet, Arie, Visualize by Das Preiing Agent Call Nin Hydrin. Anyway, if you're going to carry ammonium series amino acet, no, it won't run. Your chromatography was divided into two types, and I have studied both concepts. First, your sir, the sir's column, chromatic craft, what is the quantity? Lying down, not for Chromatography. All the children are opening up. The gates are opening. Don't move forward, child. Alright, guys. So now we move on to the second topic. Your sterile phase will also remain liquid. And what about the mobile phase? Your liquidity will remain. I mean, there's just no point here—it's a change of perspective. The bike's process is all the same. Your entire consent will be invalidated. Your sterile phase will remain liquid. You are very much the one who is loved by Anja. Jeriga for such a large amount of premium steak, certified Vera Pramaji shows the synthetic and the boat. It speaks in its own language. Liquidity movement will begin. The mobile phase will begin to move The more beautiful it is, the more it is Ability will be available with the mobile phase To bond, he quickly went up above. Partition Chromatography Chromatography And the slow pass of the seismograph and chromato graphy paper has consistently provided the basis for the entire analysis. You need to take the capillary, and take the one that remains in your mixer. 2 cm, as much sport as you want to hit, you've hit it, that's fine. Clear, let's go Let's see ahead, the next point The paper is then suspended in the chamber containing the mobile phase, okay The mobile phase rises due to capillary action, flow will occur, above the points What will it do with the points? It'll go forward at lightning speed. But the stationary phase also tries to hold it back. But no matter how hard he tries to stop, he ends up depending on that spot. If not, it will go up very rapidly. Come on Different solutes are written differently on the paper depending on their adsorption phenomena. I've just told you that already. And this developed paper strip is the chromatography similar to TLC. That's why I'm telling you about this partition chromatography. This is similar to TLC, meaning it's just like thin layer chromatography. It's clear to everyone And the last point is that if color comes, it's fine. After separation, if the color appears, it's fine. Look, it's come in here If the color comes, it's fine. No color appeared So, you'll have to show it to him in what? It will have to be visualized inside the UV chamber. You understand, don't you, kid? Come on I think now you Having attained mastery I mean, if any kind of issue arises regarding chromatography So you can clear this in a flash Retention factor First fall down Stalls Now Jaudo Sapath has arrived Who is further ahead, me or you? Who is so late, me or you? So, how does it look when shown? Paper strip That's yours, your baseline, and it's been here for just one day already. And until you, until here, run here, give me the dena, and whatever is the kulge Find out about Denavishkeng and its age Write down how far the solvent has moved from the origin point (baseline). And now divide it, and you will get what, the retention factor What will you get, retention factor? Well, I've given you a little hint already. We read this, doing it from the middle. So, carefully look, let's first see the definition The retention factor is nothing but a measure used in chromatography to describe the relative retention time of a compound compared to the solvent front. Distance traveled by the solute from the baseline divided by the distance traveled by the solvent from the baseline That is how it is represented, there is a method of representation, a symbol, which is an arya. Well, let's take a look — we're going to see a giraffe here. Look, what you just did was the stationary phase; this was the baseline. Look, this solute has dissolved up to here, and this solvent that was here has also dissolved up to here. So how will you calculate it? State the retention factor, and what does the formula indicate? Distance traveled by the solute from the baseline divided by the distance traveled by the solvent from the baseline From here, but up to here, just draw any arc, and you will get the answer. You will get the retention factor; on top of that, I had done the practical very thoroughly. If you liked the video, please share it with your friends and thank you for watching. See you in the next video, and please don't forget to subscribe at It's Practical. Thank you.