On the Antiseptic Principle of the Practice of Surgery by Joseph Lister

On the Antiseptic Principle of the Practice of Surgery. 1867. By Joseph Lister. In the course of an extended investigation into the nature of inflammation and the healthy and morbid conditions of the blood in relation to it, I arrived several years ago at the conclusion that the essential cause of superation in wounds is decomposition brought about by the influence of the atmosphere upon blood or serum retained within them, and, in the case of contused wounds, upon portions of tissue destroyed by the violence of the injury. To prevent the occurrence of superation with all its attendant risk was an object manifestly desirable, but till lately apparently unattainable, since it seemed hopeless to attempt to exclude the oxygen which was universally regarded as the agent by which putrefaction was effected. But when it had been shown by the researches of Pasteur that the septic properties of the atmosphere depended not on the oxygen or any gaseous constituent, but on minute organisms suspended in it, which owed their energy to their vitality, it occurred to me that decomposition in the injured part might be avoided without excluding the air, by applying as a dressing some material capable of destroying the life of the floating particles. Upon this principle I have based a practice, of which I will now attempt to give a short account. The material which I have employed is carbolic or phenic acid, a volatile organic compound which appears to exercise a peculiarly destructive influence upon low forms of life, and hence is the most powerful antiseptic with which we are at present acquainted. The first class of cases to which I applied it was that of compound fractures, in which the effects of decomposition in the injured part were especially striking and pernicious. The results have been such as to establish conclusively the great principle that all local inflammatory mischief and general febrile disturbances which follow severe injuries are due to the irritating and poisonous influence of decomposing blood or sloughs. For these evils are entirely avoided by the antiseptic treatment, so that limbs which would otherwise be unhesitatingly condemned to amputation may be retained, with confidence of the best results. In conducting the treatment the first object must be the destruction of any septic germs which may have been introduced into the wounds, either at the moment of the accident or during the time which has since elapsed. This is done by introducing the acid of full strength into all accessible recesses of the wound by means of a piece of rag held in dressing forceps, and dipped into the liquid. Note 1. The addition of a few drops of water to a considerable quantity of the acid induces it to assume permanently the liquid form. This I did not venture to do in the earlier cases, but experience has shown that the compound which carbolic acid forms with the blood, and also any portions of the tissue killed by its caustic action, including even parts of the bone, are disposed of by absorption and organization, provided they are afterward kept from decomposing. We are thus enabled to employ the antiseptic treatment efficiently at a period after the occurrence of the injury at which it would otherwise probably fail. Thus I have now under my care, in Glasgow Infirmary, a boy who was admitted with compound fracture of the leg as late as eight and one-half hours after the accident, in whom, nevertheless, all local and constitutional disturbance was avoided by means of carbolic acid, and the bones were soundly united five weeks after his admission. The next object to be kept in view is to guard effectually against the spreading of decomposition into the wound along the stream of blood and serum which oozes out during the first few days after the accident, when the acid originally applied has been washed out or dissipated by absorption and evaporation. This part of the treatment has been greatly improved during the past few weeks. The method which I have hitherto published, see Lancet for March 16th, 23rd, 30th and April 27th of the present year, consisted in the application of a piece of lint dipped in the acid, overlapping the sound skin to some extent, and covered with a tin cap, which was daily raised in order to touch the surface of the lint with the antiseptic. This method certainly succeeded well with wounds of moderate size, and indeed I may say that in all the many cases of this kind which have been so treated by myself or my house surgeons, not a single failure has occurred. When however the wound is very large, the flow of blood and serum is so profuse, especially during the first twenty-four hours, that the antiseptic application cannot prevent the spread of decomposition into the interior, unless it overlaps the sound skin for a very considerable distance. And this was inadmissible by the method described above, on account of the extense sloughing of the surface of the cutis which it would involve. This difficulty has however been overcome by employing a paste composed of common whiting, carbonate of lime, mixed with a solution of one part of carbolic acid and four parts of boiled linseed oil, so as to form a firm putty. This application contains the acid in too dilutive form to excoriate the skin, which it may be made to cover to any extent that may be thought desirable, while its substance serves as a reservoir of the antiseptic material. So long as any discharge continues, the paste should be changed daily, and in order to prevent the chance of mischief occurring during the process, a piece of rag dipped in the solution of carbolic acid and oil is put on next to the skin, and maintained there permanently, care being taken to avoid raising it along with the putty. This rag is always kept in an antiseptic condition, from contact with the paste above it, and destroys any germs which may fall upon it during the short time that should alone be allowed to pass in the changing of the dressing. The putty should be in a layer about a quarter of an inch thick, and may be advantageously applied rolled out between two pieces of thin calico, which maintain it in the form of a continuous sheet, which may be wrapped in a moment around the whole circumference of a limb, if this be thought desirable, while the putty is prevented by the calico from sticking to the rag, which is next to the skin. Note 2. In order to prevent evaporation of the acid, which passes readily through any organic tissue, such as the oiled silk or gutta percha, it is well to cover the paste with a sheet of block tin, or tin foil strengthened with adhesive plaster. The thin sheet lead used for lining tea chests will also answer the purpose, and may be obtained from any wholesale grocer. When all discharge has ceased, the use of the paste is discontinued, but the original rag is left adhering to the skin till healing by scabbing is supposed to be complete. I have at present in the hospital a man with severe compound fracture of both bones of the left leg, caused by direct violence, who after the cessation of the saniest discharge, under the use of the paste, without a drop of pus appearing, has been treated for the last two weeks exactly as if the fracture was a simple one. During this time the rag, adhering by means of a crust of insipidated blood collected underneath it, has continued perfectly dry, and it will be left untouched until the usual period for removing the splints in a simple fracture, when we may fairly expect to find a sound cicatrix beneath it. We cannot, however, always calculate on so perfect a result as this. More or less pus may appear after the lapse of the first week, and the larger the wound, the more likely this is to happen. And here I would desire earnestly to enforce the necessity of persevering with the antiseptic application, in spite of the appearance of superation, as long as other symptoms are favorable. The surgeon is extremely apt to suppose that any superation is an indication that the antiseptic treatment has failed, and that poulticing or water-dressing should be resorted to, but such a course would in many cases sacrifice a limb or a life. I cannot, however, expect my professional brethren to follow my advice blindly in such a matter, and therefore I feel it necessary to place before them, as shortly as I can, some pathological principles intimately connected, not only with the point we are immediately considering, but with the whole subject of this paper. If a perfectly healthy granulating sore be well washed and covered with a plate of clean metal, such as block tin, fitting its surface pretty accurately, and overlapping the surrounding skin an inch or so in every direction, and retained in position by adhesive plaster and a bandage, it will be found, on removing it after twenty-four or forty-eight hours, that little or nothing that can be called pus is present, merely a little transparent fluid, while at the same time there is an entire absence of the unpleasant odor invariably perceived when water-dressing is changed. Here the clean metallic surface presents no recesses, like those of porous lint, for the septic germs to develop in. The fluid exuding from the surface of the granulations has flowed away undecomposed, and the result is the absence of superation. This simple experiment illustrates the important fact that granulations have no inherent tendency to form pus, but do so only when subjected to preternatural stimulus. Further, it shows that the mere contact of a foreign body does not of itself stimulate granulations to superate, whereas the presence of decomposing organic matter does. These truths are even more strikingly exemplified by the fact that I have elsewhere recorded, Lancet, March 23, 1867, that a piece of dead bone free from decomposition may not only fail to induce the granulations around it to superate, but may actually be absorbed by them, whereas a bit of dead bone soaked with putrid pus infallibly induces superation in its vicinity. Another instructive experiment is to dress a granulating sore with some of the putty above described, overlapping the sound skin extensively. When we find, in the course of 24 hours, that pus has been produced by the sore, although the application has been perfectly antiseptic, and indeed, the larger amount of carbolic acid in the paste, the greater is the quantity of pus formed, provided we avoid such a proportion as would act as a caustic. The carbolic acid, though it prevents decomposition, induces superation, obviously by acting as a chemical stimulus, and we may safely infer that putrescent organic materials, which we know to be chemically accurate, operate in the same way. In so far, then, carbolic acid and decomposing substances are alike. These, that they induce superation by chemical stimulation, as distinguished from what may be termed simple inflammatory superation, such as that in which ordinary abscesses originate, where the pus appears to be formed in consequence of an excited action of the nerves, independent of any other stimulus. There is, however, this enormous difference between the effects of carbolic acid and those of decomposition. These, that carbolic acid stimulates only the surface to which it is at first applied, and every drop of discharge that forms weakens the stimulant by diluting it. But decomposition is a self-propagating and self-aggravating poison, and if it occur at the surface of a severely injured limb, it will spread into all its recesses, so far as any extravastated blood or shreds of dead tissue may extend, and lying in those recesses, it will become from hour to hour more acrid, till it requires the energy of a caustic sufficient to destroy the vitality of any tissues naturally weak from inferior vascular supply, or weakened by the injury they sustained in the accident. Hence, it is easy to understand how, when a wound is very large, the crust beneath the rag may prove here and there insufficient to protect the raw surface from the stimulating influence of the carbolic acid in the putty. And the result will be first the conversion of the tissue so acted on into granulations, and subsequently the formation of more or less pus. This, however, will be merely superficial, and will not interfere with the absorption and organization of extravastated blood or dead tissues in the interior. But, on the other hand, should decomposition set in before the internal parts have become securely consolidated, the most disastrous results may ensue. I left behind me in Glasgow a boy, thirteen years of age, who between three and four weeks previously met with a most severe injury to the left arm, which he got entangled in a machine at a fair. There was a wound six inches long and three inches broad, and the skin was very extensively undermined beyond its limits, while the soft parts were generally so much lacerated that a pair of dressing forceps introduced at the wound, and pushed directly inwards, appeared beneath the skin at the opposite aspect of the limb. From this wound several tags of muscle were hanging, and among them was one consisting of about three inches of the triceps and almost its entire thickness, while the lower fragment of the bone, which was broken high up, was protruding four inches and a half, stripped of muscle, the skin being tucked in under it. Without the assistance of the antiseptic treatment, I would certainly have thought of nothing else but amputation at the shoulder joint, but as the radial pulse could be felt, and the fingers had sensation, I did not hesitate to try to save the limb, and adopted the plan of treatment above described, wrapping the arm from shoulder to below the elbow in the antiseptic application, the whole interior of the wound, together with the protruding bone, having previously been freely treated with strong carbolic acid. About the tenth day, the discharge, which up to that time had been only sanius and cirrus, showed a slight admixture of slimy pus, and this increased till, a few days before I left, it amounted to about three drachms in twenty-four hours. But the boy continued as he had been after the second day, free from unfavourable symptoms, with pulse, tongue, appetite and sleep natural, and strength increasing, while the limb remained as it had been from the first, free from swelling, redness or pain. I therefore persevered with the antiseptic dressing, and before I left the discharge was already somewhat less, while the bone was becoming firm. I think it likely that, in that boy's case, I should have found merely a superficial sore, had I taken off all the dressings at the end of the three weeks, though, considering the extent of the injury, I thought it prudent to let the month expire before disturbing the rag next the skin. But I feel sure that, if I had resorted to ordinary dressing when the pus first appeared, the progress of the case would have been exceedingly different. The next class of cases to which I have applied the antiseptic treatment is that of abscesses. Here also the results have been extremely satisfactory, and in beautiful harmony with the pathological principles indicated above. The pyogenic membrane, like the granulations of a sore, which it resembles in nature, forms pus, not from any inherent disposition to do so, but only because it is subjected to some preternatural stimulation. In an ordinary abscess, whether acute or chronic, before it is opened, the stimulus which maintains the superation is derived from the presence of pus pent up within the cavity. When a free opening is made in the ordinary way, this stimulus is got rid of, but the atmosphere gaining access to the contents, the potent stimulus of decomposition comes into operation, and pus is generated in greater abundance than before. But when the evacuation is effected on the antiseptic principle, the pyogenic membrane, freed from the influence of the former stimulus, without the substitution of a new one, ceases to superate, like the granulations of a sore under metallic dressing, furnishing merely a trifling amount of clear serum, and whether the opening be dependent or not, rapidly contracts and coalesces. At the same time, any constitutional symptoms previously occasioned by the accumulation of the matter are got rid of, without the slightest risk of the irritative fever or hectic hitherto so justly dreaded in dealing with large abscesses. In order that the treatment may be satisfactory, the abscess must be seen before it is opened. Then, except in very rare and peculiar cases, there are no septic organisms in the contents, so that it is heedless to introduce carbolic acid into the interior. Indeed, such a procedure would be objectionable, as it would stimulate the pyogenic membrane to unnecessary superation. All that is requisite is to guard against the introduction of living atmospheric germs from without, at the same time that free opportunity is afforded for the escape of the discharge from within. Note 3. As in the instance of one of these exceptional cases, I may mention that of an abscess in the vicinity of the colon, and afterwards prove by postmortem examination to have once communicated with it. Here the pus was extremely offensive when evacuated, and exhibited vibrios under the microscope. I have so lately given elsewhere a detailed account of the method by which this is effected Lancet, July 27, 1867, that I shall not enter into it at present further than to say that the means employed are the same as those described above for the superficial dressing of compound fractures. These, a piece of rag dipped into the solution of carbolic acid and oil to serve as an antiseptic curtain, under cover of which the abscess is evacuated by free incision, and the antiseptic paste to guard against decomposition occurring in the stream of pus that flows out beneath it, the dressing being changed daily until the sinus is closed. The most remarkable results of this practice in the pathological point of view have been afforded by cases where the formation of pus depended on disease of bone. Here the abscesses, instead of forming exceptions to the general class in the obstinacy of the superation, have resembled the rest in yielding in a few days only a trifling discharge, and frequently the production of pus has ceased from the moment of the evacuation of the original contents. Hence it appears that caries, when no longer laboring as heretofore under the irritation of decomposing matter, ceases to be an opprobrium of surgery, and recovers like other inflammatory infections. In the publication before alluded to, I have mentioned the case of a middle-aged man with a psoas abscess depending in diseased bone, in whom the sinus finally closed after months of patient perseverance with the antiseptic treatment. Since that article was written, I have had another instance of abscess equally gratifying, but the differing in the circumstance that the disease and the recovery were more rapid in their course. The patient was a blacksmith, who had suffered four and a half months before I saw him, from symptoms of ulceration of cartilage in the left elbow. These had laterally increased in severity, so as to deprive him entirely of his night's rest and of appetite. I found the region of the elbow greatly swollen, and on careful examination found a fluctuating point at the outer aspect of the articulation. I opened it on the antiseptic principle, the incision evidently penetrating to the joint, giving exit to a few drachms of pus. The medical gentleman under whose care he was, Dr. McGregor of Glasgow, supervised the daily dressing with the carbolic acid paste till the patient went to spend two or three weeks at the coast, when his wife was entrusted with it. Just two months after I opened the abscess, he called to show me the limb, stating that the discharge had been, for at least two weeks, as little as it was then, a trifling moisture upon the paste, such as might be accounted for by the little sore caused by the incision. On applying a probe guarded with an antiseptic rag, I found that the sinus was soundly closed, while the limb was free from swelling or tenderness, and although he had not attempted to exercise it much, the joint could already be moved through a considerable angle. Here the antiseptic principle had affected the restoration of a joint, which, on any other known system of treatment, must have been excised. Ordinary contused wounds are, of course, amenable to the same treatment as compound fractures, which are a complicated variety of them. I will content myself with mentioning a single instance of this class of cases. In April last, a volunteer who was discharging a rifle when it burst, and blew back the thumb with its metacarpal bone, so that it could be bent back as on a hinge at the trapezial joint which had evidently been opened, while all the soft parts between the metacarpal bones of the thumb and forefinger were torn through. I need not insist before my present audience on the ugly character of such an injury. My house surgeon, Mr. Hector Cameron, applied carbolic acid to the whole raw surface, and completed the dressing as if for a compound fracture. The hand remained free from pain, redness, or swelling, and, with the exception of a shallow groove, all the wound consolidated without a drop of matter, so that, if it had been a clean cut, it would have been regarded as a good example of primary union. The small, granulating surface soon healed, and at present a linear cicatrix alone tells of the injury he has sustained, while his thumb has all its movements, and his hand a fine grasp. If the severest forms of contused and lacerated wounds heal thus kindly under the antiseptic treatment, it is obvious that its application to simple incised wounds must be merely a matter of detail. I have devoted a good deal of attention to this class, but I have not as yet pleased myself altogether with any of the methods I have employed. I am, however, prepared to go as far as to say that a solution of carbolic acid in twenty parts of water, while a mild and cleanly application, may be relied on for destroying any septic germs that may fall upon the wound during the performance of an operation, and also that, for preventing the subsequent introduction of others, the paste above described, applied as for compound fractures, gives excellent results. Thus I have had a case of strangulated inguinal hernia, in which it was necessary to take away half a pound of thickened omentum, heal without any deep-seated superation, or any tenderness of the sac, or any fever, and amputations, including one immediately below the knee, have remained absolutely free from constitutional symptoms. Further, I have found that when the antiseptic treatment is efficiently conducted, ligatures may be safely cut short, and left to be disposed of by absorption or otherwise. Should this particular branch of the subject yield all that it promises, should it turn on further trial, that when the knot is applied on the antiseptic principle, we may calculate as securely as if it were absent on the occurrence of healing, without any deep-seated superation. The delegation of main arteries in their continuity will be deprived of the two dangers that now attend it, these, those of secondary hemorrhage, and an unhealthy state of the wound. Further, it seems not unlikely that the present objection to tying an artery, in the immediate vicinity of a large branch, may be done away with, and that even the inominate, which has lately been the subject of an ingenious experiment by one of the Dublin surgeons, on account of its well-known fatality under the ligature for secondary hemorrhage, may cease to have this unhappy character, when the tissues in the vicinity of the thread, instead of becoming softened through the influence of an irritating, decomposing substance, are left at liberty to consolidate firmly, near an unoffending, though foreign body. It would carry me far beyond the limited time which, by the rules of the association, is alone at my disposal. Were I to enter into the various applications of the antiseptic principle in the several special departments of surgery, there is, however, one point more, that I cannot but advert to, these, the influence of this mode of treatment upon the general healthiness of a hospital. Previously, to its introduction, the two large wards, in which most of my cases of accident and of operation are treated, were among the unhealthiest in the whole surgical division of the Glasgow Royal Infirmary, in consequence, apparently, of those wards being unfavourably placed with reference to the supply of fresh air, and I have felt ashamed, when recording the results of my practice, to have so often to allude to hospital grand green, or paemia. It was interesting, though melancholy, to observe, that whenever all, or nearly all, the beds contained cases with open sores, these grievous complications were pretty sure to show themselves, so that I came to welcome simple fractures, though in themselves of little interest, either for myself or the students, because their presence diminished the proportion of open sores among the patients. But since the antiseptic treatment has been brought into full operation, and wounds and abscesses no longer poison the atmosphere with putrid exhalations, my wards, though in other respects under precisely the same circumstances as before, have completely changed their character, so that during the last nine months, not a single instance of paemia, hospital gangrene, or erysipelas has occurred in them. As there appears to be no doubt regarding the cause of this change, the importance of the fact can hardly be exaggerated. End of On the Antiseptic Principle of the Practice of Surgery by Joseph Lister