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This video demonstrates laboratory experiments to identify the presence of carbohydrates, proteins, and fats in various food samples, specifically tailored for 12th-grade practical examinations.
Hello friends and welcome to my channel.
So, friends, I am Sachin Shukla, and you are watching A2J Practical.
So, friends, today's experiment is the 12th practical.
Experiment Number 9, the study of carbohydrates, fats, and proteins.
We are going to see these three types of tests, which you will encounter in experiment number 19.
And basically what we are going to do here is test different food samples or materials.
and check which one contains carbohydrates, which one contains fats, and which one contains oil, and which one contains proteins.
So this is what we are going to do here.
So let me begin the practical part and show you.
Whatever answers you have, you will receive them next to me after some time.
You can also see what is written.
And if you want to understand the experiment, then you can also understand the experiment.
which you have to carry out on the day of the board practical.
So let me start it.
So, friends, I've placed the test tube holder here.
What you have to do is, whatever sample or material is provided to you, the substance is provided.
This is my essence.
You have to take it this way.
And the first test that has been mentioned is that you must add water to the substance.
So I put a little water in it here.
And what I observe is that it is not dissolving.
Is it dissolving?
Basically, starch can be present in it.
You can see it as white; it is not colorless.
See, this is completely soluble.
So basically, what could happen here?
Starch may be present.
So let's do another test here for the starch test.
Let's perform the iodine test here.
For this, I will take another test tube here.
Here I have that test tube.
In this, I take the substance that is present.
And later I will add a few drops of iodine to it.
So I took the substance here.
Clear.
Whatever substance is given to me, I have brought it here.
And here I am going to add a few drops of iodine.
I will do this.
And after adding the iodine solution, what should appear?
Blue color.
If the blue color appears, then starch is present.
If not, then abstain from friends.
You see, this is iodine.
And I'm going to add this to it.
And this is added.
So you see, this is the blue color.
Basically, this is blue.
So what's in this?
Friends, iodine is present.
So you can easily do this test.
And you can easily see the result of this on my website.
The given sample of the food-safe material contains carbohydrates.
What is our starch?
It is a type of carbohydrate.
Clear.
Your first test is complete here.
We are going to perform the next test.
Which is provided by protein.
That protein will be present in any substance.
So how can we find out easily?
So a sample has been given to me.
Which is protein.
But I'm telling you the result directly.
So what are you doing about it?
You have to take a substance.
And add 10% NaOH to it.
Here I have 10% NaOH.
What am I supposed to do with this?
I will add it.
This 10% is extremely strong.
Clear.
I have added 10% NaOH to it.
What to put?
1% copper sulfate solution.
So I have made this copper sulfate solution.
1%
You will receive it in your table area.
You have to add this to it.
And as soon as you add it to it.
You see the solution of the blue color.
Bluish violet.
So this is basically.
You can also see it by heating it.
But it comes in a very pure color.
Which you are beginning to see here.
So you know what protein is here.
Present.
Clear.
You can also achieve very good color by heating it.
In the next test.
I mean in the second part of this.
I'll show you.
In which it is placed.
Neanhydrin solution.
What will happen if you perform the Neanhydrin test?
Then what will happen?
So you have to do the same.
In the substance.
This time you have to use dilute NaOH.
Now I am telling you about this protein test in two ways.
So you have to add this diluted NaOH to it.
Clear.
And now what else do you have to add to it?
Neanhydrin solution.
So, friends.
You have to add a small amount of Neanhydrin solution to it.
Clear.
Now after adding a small amount of Neanhydrin solution to it.
What do you have to do, friend?
Heat it.
If it is a vivid blue color.
Will come on cooling.
Clear.
I'll heat this.
If you put it to cool it.
You will get this blue color.
Which I will show you later.
I heat it first.
Now, after some time, it will turn blue.
I keep it here.
The next test is our fat test.
Whose test is it?
Fat.
Fat or oil.
So I have oil and fats. thuật ngữ tiếng Anh cho "dầu và mỡ" là "oil and fats".
What is in this?
Friends.
You are given fats or oil, just as you are given this; you need to take a small amount of it.
Clear? You need to take a little of this.
And water should be sprinkled here.
So, this will basically be immiscible in water, as you can see?
Now I'll go for the offset, potassium bisulfate
Here it is, my potential by sulphate
Now in this, I add a few drops of potassium sulfate
If my bass isn't dropping, then I'm just crystal-clear here, right here.
And we shake it slightly.
And let's heat it up a little.
Lying down, you will see the test results for cholesterol, fat, and protein.
So, you're already getting these results.
You also know how to do it.
I have already told you.
You will see this answer here, and if you wish, you can write even more than this.
So thank you for watching this practical experiment
The very explosive experiment that I carry out
So you can also see it; his name is a game's title.
Plik, the rice field