To study the effect of enzymes on starch egg albumin and fat #11thbiologypractical

So, hey guys and welcome to my channel, friends. Myself Shashi Shukla and you are watching ATJ Practicals. Friends, today I'm starting the 11th Grade Biology Practicals. This is your experiment number 10. To study the effect of Enzyme, Albumins, Antigens Proteins, Fat So, what are the effects of Enzyme on all this? We are going to see all those things here. This is your experiment number 10. Students of 11th Biology, watch carefully. There is one more announcement. All the practicals of Physics and Chemistry are available here. 11th as well as 12th. Go take a look at it. All the practicals of Physics, Chemistry, and Biology are being uploaded. So, watch it. If you like it, share it with your friends. So, let's begin your Experiment Number 10. So, first of all, You have to prepare 3 test tubes. So, in the first test tube, you have to add 5 ml of starch. So, I'll add 5 ml of starch to the first test tube. Add 5 ml of starch and 3 ml of buffer solution in each test tube. Keep both the constants that are 5 ml of starch and 3 ml of buffer solution in each test tube Now, I'll add 3 ml of buffer solution in each test tube. So, now I have the buffer solution. So, I have to add 3 ml. Now, I am to add the buffer solution to the first test tube. Now, I have added 3 ml of buffer solution to the first test tube. Now, I will make Here are those who are preparing for the entire process Now you have to put it into a test tube 3 ml celery amylase and in the other one, 3 ml distilled water And in the other one, you have to insert it. 3 ml of distilled water and 10 drops of SCl I need to go first in the previous one. 3 ml celery amylase Now, this server mail is correct. This is the mouth part, okay? How can you even make this, as written in your handbook? Alright, so how much do you need? This is 3 ml. So I've finally taken 3 ml here. 3 ml You must take the Selverry Amailaj. So you don't have an Amailaj either So I have to feint in the first one. Then what should you do next? Take 3 ml of distilled water. Distilled water I will give you ten. 3 ml And in the last one, what should I put? Just a little bit of distilled voter to add 3 ml distilled water and 10 drops of SCL So 3 ml of distilled water I'll cover first, and later I'll I will take 10 drops of SCL Alright, now I'm in this I will take 10 drops of SCL One, two Three, fourth After bypassing these people What do you need to do? Basically, you Keep it at 37 degrees Celsius 10 to 15 minutes Alright, and later we will include this in They will apply the Lugol's iodine solution. Lugol's iodine solution will be added here. What am I doing? I'm keeping the gas very, very slow. And here I've stuck the thermometer. So that I can get the temperature of this thing, what is it 37, which I can maintain here OK? I need to maintain this up to about 15 meters. First, you drop some I will immerse the people in the iodine solution. And I'll see what all I have This observation is available here. So, let's see what I do with Lugol's iodine solution I add drop by drop into each test tube. If Amber coloration appears now So let's see what's in this Amber color comes now So if amber color comes in it, in that case No presence of stars So first, we have test tube no. 1 I have written "one" in it, so I check "one" first. So how is this coming out for us? amber color Now we add in the second one. So first Here you are seeing You've started getting blue color. So, what is this dark blue color? remains undigested Alright, what is this? represents that which remains undigested Alright, now let's do the third one. Which one do we see in the third one? The color comes in the third one, these luggans Alright, and what about looking at it? color now comes so that is your It's getting dark blue, okay So these are three tests. that will represent that you are It has started digesting, this is not Now, your observation on my sitar You must be feeling bloated, right? Okay. Now with his help, you can write one of yours. The second blue is coming. Dark blue and the last one are also coming as dark blue for you You can see these three test tubes here, okay? It's coming only in dark blue. So that is the observation. inference and calculation, okay So, friends, I'm conducting the second experiment. Effect of pepsin on egg white So we have this egg white solution here And I have pepsin here with me. So, we need to make a thin test tube, okay? The thin test tube. And whatever solution you make will be in a test tube. Not 37 degrees Celsius to you either. If you need to maintain temperature, then please fill in my details There's nothing, well, apricots, but I'm something Daru T Sel So, Philal Min, give me 5. Whatتنوع So it's in 5. Daru SCl Me in 3 Pepsin solution is I surrender. 3 ml of pepsin solution 2 ml Raj Daroot Cell Nikyaō And in the last one Dilute 3 ml of pepsin solution. And 2 ml of NaOH, this needs to be diluted. 3 ml of pepsin solution solution And add 2 ml of NaOH. So, this is NaOH that I have here. So how much should I take? 2 ml So, what should I do with 2 ml? NaOH solution This is my third test tube, it's a cheek swab. What do you need to do now? To solve this up to Thierno Test Tube at 37 Degrees I saw it at Celsius. This is the Bhavatara; its tamburachchhara 37 degrees Celsius What should I do with this now? Sara Solis gets it. I will place it. test tube no. 1, test tube no. 2 test tube no. 3 In 30 minutes, what will I do? I'll hold onto this. Here are 2 results. inference, yes, my test tube number 1 test tube no. 1 only SCL that and this milky white color The raha hai, so that's why we can say it here. What are pepsin and the proteins of amara? dumped means our protein is not digested Test tube No. 2 contained our pepsin, and As you can see, the clear solution has turned out to be non-milky. So what has happened to our protein here? It has been digested. Okay? And Enin Teshcheb Number Three, Enivach, and Pepsin were it. I'm summarizing it for you After adding 3 ml of distilled water What will I see here? There is a separation between two layers. The water separated, and ours What happened to the oil? It got separated. So our layer of water is different, and The oil layer is separate now. Now we move on to the next one. This is exactly what we test in a test tube. We've taken this already; now we'll be cutting corners on it. bile juice and They dodge droplets Kalmukuljan With this, Precisix, Kemestu per Go with the practical one. We will subscribe to this channel. Do you like it? So, please subscribe to this channel. What should we do and share from Jadhav to the people of Jadhav? Thank you for watching this video and please do subscribe to A2Jit Practical. Thank you