Separation Of Plant Pigments by paper chromatography || 12th Biology practical @a2zpractical991

Yes, in the Gireen part This is a leaf. yu means leaf to separate pigments When I am your shadow, the great Shimon Sejanus Separate the two pigments You can see, these are all the people. When the noose tightened around Balaji at 12 to experiment 2 Shhering pigment Shao Gang Si Jian Du played From Thara, I took the Hamsa Raspi Riri Line. Cars that have been parked for two to three years You're weighing 2, what do you do with it for yourself? If two monks are with you, they will be monks for you; three monks will be monks for you. So, with the chromatography technique, what can we do with it, separate it? So today we will see how we can separate plant pigments from leaves Separation of plant pigments by using paper chromatography These are the very practicals, and they will be performing these very experiments. For this, children, you'll need some leaves because inside the leaves are your pigments, which are present there. Now, let's see exactly what I'm going to do with this leaf. I'm going to crush this leaf by putting it into the grinder and the pestle. After precipitation, I will dissolve it in a mild acetone and Mg-saturated buffer solution, and then precipitate it again. Later, I will filter these pigments through the filter paper. And we'll see how many different types of bands or pigments here这不是在滤 catalogue 上看到的 This is my leaf, and from this leaf, we need to show the pigments on top of the filter paper. First, what you need to do is, you can basically take whichever leaf you want to take. And you'll have to do it very well—crush it. But you'll have to mix in a little aceto, and for the buffer solution, I'll use this. MgCl2 Okay, so everything is finally done; I've ground this using this grinder, okay? And since it contains What should we do with this? Should we filter it? We'll catch it, it'll become thin, it'll be fucked, but we need to find it out, DSP. But what should we do with the palindroma pigmynt? We need to find it out like this. You brought this filter to me, now what should I do? I'll get a filter paper, and What should I do with a centimeter above it? I'll spot you, okay This filter paper also serves as a stationary phase. And what are we going to do about it, the one who's supposed to be watching over the plant? Looking at the separation of lorophyll Now, I will draw a line exactly 3 cm above this. What am I going to do here, and what am I going to do here? Now, right here, what am I going to do, those who strike spots Who is the one who's been spotting me, whom I need to find out? What is present in this separation? We keep hitting the spot for this here. Later, we will place it in a gas jar. in which our mobile phase is present Now I'll take these liquids here, broken-like in the capillary I will take these liquids into the capillary. What am I supposed to do here? I'll hit a spot right in the middle Just a little of this soup will go, then I'll take a spot I'll beat you up here. By then, I'll show you the mobile phase. How would you prepare the mobile phase? for preparing the mobile phase Kids, you need to go get the gas. And in it, you acetone and petroleum ether Add a ratio, that is 90 ratio 10 I have petroleum ether here. I'll take 20 ml Here you go. 2 or 3 ml Alright I take 2 or 3 ml of it. Alright Come on, now this Petroleum ether is highly flammable. Alright, fire can break out immediately in this. If this gingerbread comes into contact with fire, it's okay. What should we do now? Let's take a look at this filter paper now. So I do a job. As it is liquid The side move above will go from the baseline. The line I drew is the base line; this liquid inside it is called Mobile face and this is your Eja stationary face working The filter paper you have, okay, let's take a look then